ELECTRON MICROSCOPIC IMMUNOCYTOCHEMISTRY: A REVIEW

Immunocytochemistry at the Electron-Microscopic Level

Light and electron microscopic immunocytochemistry of the pituitary gland of the tortoise.

The pars distalis of the tortoise, Geoclemis reevesii, was studied immunocytochemically with regards to its fine structure and distribution of different cell types. The pars distalis consists of cephalic and caudal lobes and a zona tuberalis, each with distinct cellular components. The cephalic lobe contains ACTH cells and prolactin (PRL) cells, while the caudal lobe consists of STH (GH) cells,...

Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level.

Nucleoli are the morphological expression of the activity of a defined set of chromosomal segments bearing rRNA genes. The topological distribution and composition of the intranucleolar chromatin as well as the definition of nucleolar structures in which enzymes of the rDNA transcription machinery reside have been investigated in mammalian cells by various immunogold labelling approaches at the...

Electron microscopic immunocytochemistry of interstitial retinol-binding protein in vertebrate retinas.

Interstitial retinol binding protein (IRBP) is a soluble glycoprotein found in the interphotoreceptor matrix (IPM) and implicated in shuttling retinol between retina and pigment epithelium (PE) cells. The authors have studied the distribution of IRBP by EM immunocytochemistry. Thin sections of Lowicryl K4M embedded R. pipiens, X. laevis, bovine and human retinas were labeled sequentially with a...

Electron microscopic immunocytochemistry by the protein A-gold method of two types of rat anterior pituitary ACTH cells.

Immunocytochemical detection of ACTH using the protein A-gold method was performed in the adult male rat anterior pituitary gland after simple glutaraldehyde fixation and double fixation with glutaraldehyde and osmium tetroxide. Immunoreactive ACTH cells of the rat anterior pituitary can be classified into two groups: typical and atypical ACTH cells. The typical ACTH cells are small stellate ce...